HER2 Antibody / ErbB2

• Catalog Number: orb2638129
• Category: Antibodies
• Description: ErbB2, also called HER2, is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. This ErbB2 antibody shows minimal cross-reaction with other members of the family. The protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ERB2/776
• Tested applications: ELISA, FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein (intracellular domain) was used as the immunogen for this HER-2 antibody. (2)
• Antibody Type: Primary Antibody
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This HER-2 antibody is available for research use only.
• Entrez: 2064
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER-2 antibody to be titered up or down for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.2. This HER2 antibody is specific to the intracellular region of the protein.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of MeOH-fixed human MCF7 cells with HER-2 antibody (clone ERB2/776, green) and Reddot nuclear stain (red).

Immunofluorescent staining of PFA-fixed human MCF7 cells with HER-2 antibody (clone ERB2/776, green) and Reddot nuclear stain (red).

Flow cytometry testing of permeabilized human MCF7 cells with HER-2 antibody (clone ERB2/776); Red = isotype control, Blue = HER-2 antibody.

SDS-PAGE analysis of purified, BSA-free HER-2 antibody (clone ERB2/776) as confirmation of integrity and purity.