HER2 Antibody / ErbB2

• Catalog Number: orb1825175
• Category: Antibodies
• Description: C-erbB-2/HER-2 is a member of the EGFR family. This mAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant partial protein sequence (within amino acids 1055-1255) from the human protein was used as the immunogen for the recombinant HER2 antibody.
• UniProt ID: P04626
• Tested applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Antibody Type: Primary Antibody
• Clone Number: ERBB2/8142R
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant HER2 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human breast carcinoma tissue with recombinant HER2 antibody (clone ERBB2/8142R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma tissue with recombinant HER2 antibody (clone ERBB2/8142R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.