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Catalog Number | orb1822459 |
---|---|
Category | Antibodies |
Description | Mouse Anti-Yeast HDEL Monoclonal IgG2b |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2E7 |
Tested applications | ICC, IF, WB |
Reactivity | Drosophila, Plant, Saccharomyces, Yeast |
Isotype | IgG2b |
Immunogen | Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
Conjugation | Unconjugated |
MW | 78kDa |
Target | HDEL |
Storage | -20°C |
Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
Alternative names | H-D-E-L (his-asp-glu-leu) Antibody, endoplasmic re Read more... |
Note | For research use only |
Application notes | 1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
ICC, IF, IHC | |
Bovine, Canine, Drosophila, Frog, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep | |
Mouse | |
Monoclonal | |
APC |
ELISA, ICC, IF, IHC, WB | |
Bovine, Canine, Drosophila, Frog, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep | |
Mouse | |
Monoclonal | |
Biotin |
ICC, IF, IHC | |
Bovine, Canine, Drosophila, Frog, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep | |
Mouse | |
Monoclonal | |
FITC |
ELISA, IHC, WB | |
Bovine, Canine, Drosophila, Frog, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep | |
Mouse | |
Monoclonal | |
HRP |
ELISA, ICC, IF, IHC, WB | |
Bovine, Canine, Drosophila, Frog, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep | |
Mouse | |
Monoclonal | |
PerCP |