Growth hormone receptor Rabbit Polyclonal Antibody

• Catalog Number: orb10712
• Category: Antibodies
• Description: Growth hormone receptor Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GHR (101-200/451aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: GHR
• UniProt ID: P10912
• RRID: AB_10747968
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GH receptor; GHBP; GHR; Growth hormone receptor pr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhao, Yong et al. Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production Theriogenology, 89, 280-288 (2017)

PubMed: 28043364

Blank control: A549 (blue), the cells were fixed with 2% paraformaldehyde (10 min). Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 5 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Positive control: A549 cells, Concebtration: 1 µg/10^6 cells, Incubation conditions: Avoid light, 30 minutes on the ice.

Sample: Lane 1: Testis (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Testis (Rat) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Growth hormone receptor (orb10712) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130/110 kD, Observed band size: 130 kD.

Paraffin embedded tammar wallaby (Macropus eugenii) liver labeled with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated (orb10712) at 1:300 followed by conjugation to a secondary antibody and staining.