GRK2 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1172543
• Category: Antibodies
• Description: GRK2 Recombinant Rabbit Monoclonal Antibody
• Target: GRK2
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG/Kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1:50-100, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: D3F9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Adrenergic Receptors, Cell Biology, G Protein-Coup
• Note: For research use only

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:5000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Mouse brain, Rat brain, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 80 kDa, Observed MW: 80 kDa.

Cell line: U87-MG, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172543.