Goat IgG F(ab')2 Antibody

• Catalog Number: orb346982
• Category: Antibodies
• Description: Goat IgG F(ab')2 antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Goat
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.6 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG, Goat IgG F(ab’)2 and Goat Serum. No reaction was observed against Goat IgG F(c).
• Immunogen: Goat IgG F(ab')2 fragment
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:275,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:25,000
• Application notes: Anti-Goat IgG F(ab')2 antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: rabbit anti-Goat IgG F(ab')2 Antibody, rabbit anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA Results of Rabbit Anti-Goat IgG F(ab')2 Antibody tested against purified Goat IgG F(ab')2. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n orb2652767) [Green Line] and Goat IgG F(ab')2 (p/n orb2652764) [Purple Line]. The working dilution is 1:38000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer Donkey Anti-Goat IgG HRP conjugated (p/n orb347032) and TMB substrate (p/n orb348651).