Goat anti-Sp1 Antibody

• Catalog Number: orb1523884
• Category: Antibodies
• Description: Sp1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 735 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Sp1
• UniProt ID: P08047
• NCBI: NP_612482.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: specificity protein 1; transcription factor Sp1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse Sp1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-Sp1 antibody orb1523884 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Sp1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , and Jurkat (50µg) cells. Antibodies: Affinity purified goat anti-Sp1 antibody orb1523884 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Sp1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified goat anti-Sp1 antibody orb1523884 used for IP at 6 µg/mg lysate.

Detection of mouse Sp1 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified goat anti-Sp1 (Cat. No. orb1523884) used at a dilution of 1: 1, 000 (1 µg/ml). Detection: DAB

Detection of human Sp1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-Sp1 (Cat. No. orb1523884) used at a dilution of 1: 5, 000 (0.2 µg/ml). Detection: DAB