Goat anti-Rat IgG (H&L), TRITC conjugated

• Catalog Number: orb700145
• Category: Antibodies
• Description: Goat polyclonal antibody against Goat anti-Rat IgG (H&L) conjugated to TRITC
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: TRITC
• Reactivity: Rat
• Form/Appearance: Lyophilized
• Purity: Immunogen affinity purified IgG.
• Purification: This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis. Please include 5 % goat serum in secondary antibody diluent buffer. Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
• Dilution range: The optimal working dilution should be determined by the investigator, Usually 1 : 100-1 : 500,
• Application notes: This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis. Please include 5 % goat serum in secondary antibody diluent buffer. Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
• Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
• Note: For research use only