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Catalog Number | orb19943 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to PITX3 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Dilution range | ELISA: 1:1000, WB: 0.3-1 μg/ml |
Conjugation | Unconjugated |
MW | 31.8 |
Target | PITX3 |
Entrez | 5309 |
Protein Sequence | PEHGCKGQEHSD |
RRID | AB_10750434 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti PITX3 antibody, anti paired-like homeodomain Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx 40kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 31.8kDa according to NP_005020.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Coulon et al, J Biol Chem. 2007 Nov 9;282(45):33192-200.; PMID: 17848564). Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19943 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.