Goat anti-PARK7 / DJ-1 Antibody

• Catalog Number: orb18461
• Category: Antibodies
• Description: Goat polyclonal antibody to PARK7
• Target: PARK7 / DJ-1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: AAQVKAPLVLKD
• RRID: AB_10751876
• MW: 19.9
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.05-0.1 μg/ml, IHC-P: 5 μg/ml
• Application notes: Approx 20kDa band seen in Jurkat lysate [Predicted MW of approx. 20kDa according to NP_009193]. Recommended for use at 0.5-1 μg/ml.ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PARK7; DJ1; DJ-1; Parkinson disease (autosomal rec
• Note: For research use only
• Entrez: 11315

orb18461 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18461 (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb18461 (0.001ug/ml) staining of Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemilumines

Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml orb18461 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.