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| Catalog Number | orb323031 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to NR5A2 |
| Target | NR5A2 / LRH1 (aa328-338) |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | QEQANRSKHEK |
| MW | 61.3; 56.5 |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml |
| Application notes | WB: Approx 55kDa band observed in lysates of cell line HepG2 (calculated MW of 56.5kDa according to NP_003813.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Placenta. Recommended concentration: 5µg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti NR5A2 antibody, anti nuclear receptor subfami Read more... |
| Note | For research use only |
| Entrez | 2494 |
| Expiration Date | 12 months from date of receipt. |

Western blot analysis of HepG2 lysate using NR5A2 antibody

Immunohistochemical staining of Human Placenta using NR5A2 antibody
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