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Goat anti-ITK Antibody
Catalog Number: orb137166
| Catalog Number | orb137166 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal to ITK |
| Target | ITK |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Bovine, Human, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | KERPEDRPAFSR |
| MW | 71.8 |
| Tested applications | ELISA, FC, IF, IHC, WB |
| Dilution range | ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75ug/ml |
| Application notes | WB: Approx 70kDa band observed in Rat Colon, Lung and Spleen lysates (calculated MW of 72.4kDa according to Rat NP_001102295.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of Jurkat cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concen |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti ITK antibody |
| Note | For research use only |
| Entrez | 3702 |
Western blot analysis of Rat Thymus lysate using ITK antibody
Immunohistochemical staining of Human Spleen using ITK antibody
Flow cytometric analysis of paraformaldehyde fixed Jurkat cells using ITK antibody.
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