Goat anti-CD68 Antibody

• Catalog Number: orb318736
• Category: Antibodies
• Description: Goat polyclonal antibody to CD68
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 37.4; 34.7
• Target: CD68
• Entrez: 968
• Protein Sequence: PNKTKVQGSCEGAH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CD68 antibody, anti CD68 molecule antibody, a
• Note: For research use only
• Application notes: WB: Approx 110kDa band observed in lysates of cell line U937 (calculated MW of 37.4kDa according to NP_001242.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3µg/ml.
• Expiration Date: 12 months from date of receipt.

2 μg/ml staining of U937 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

5 μg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.