APE1/APEX1 Antibody

• Catalog Number: orb18393
• Category: Antibodies
• Description: Goat polyclonal antibody to APEX1
• Target: APE1 / APEX1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PKRGKKGAVAEDGD
• RRID: AB_10755395
• MW: 35.6
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 4-6 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in Human epidermoid fibroblast A431 and Human cervix epitheloid fibroblast HeLa lysates (calculated MW of 35.6kDa according to NP_001632.2; NP_542379.1; NP_542380.1. Recommended concentration: 0.5-2 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti APE1 antibody, anti APEX1 antibody, anti APEX
• Research Area: Cancer, Cardiovascular Disease, DNA Damage, Epigen
• Note: For research use only
• Entrez: 328
• Expiration Date: 12 months from date of receipt.

orb18393 staining (0.5ug/ml) of A431 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot analysis of A431 (Lane 1), HeLa (Lane 2) and MCF7 (Lane 3) nuclear lysates using APEX1 antibody

Immunohistochemical staining of Human Breast using APEX1 antibody