ACHE Antibody

• Catalog Number: orb19007
• Category: Antibodies
• Description: Goat polyclonal antibody to ACHE
• Target: ACHE
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QFDHYSKQDRCSDL
• RRID: AB_10749477
• MW: 67.8; 54.9
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 67.8kDa according to NP_000656.1). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ACHE antibody, anti YT antibody, anti acetylc
• Research Area: Epigenetics, Neuroscience
• Note: For research use only
• Entrez: 43
• Expiration Date: 12 months from date of receipt.

orb19007 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of Human Brain (Cerebellum) lysate using ACHE antibody

Western blot analysis of HepG2 (A), Daudi (B) and (0.3ug/ml) HeLa (C) and Jurkat (D) cell lysate using ACHE antibody

Immunofluorescence analysis of U2OS cells using ACHE antibody

Flow cytometric analysis of HeLa cells using ACHE antibody