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Item 1 of 4
Item 1 of 4
Goat anti-58KGolgi protein(Internal)/FTCD Antibody
Catalog Number: orb18891
| Catalog Number | orb18891 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to FTCD |
| Target | 58KGolgi protein(Internal)/FTCD |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Bovine, Human, Mouse, Porcine, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | CLREQGRGKDQPGRL |
| RRID | AB_10767143 |
| MW | 58.9; 58.9; 61.3 |
| Tested applications | ELISA, FC, IF, WB |
| Dilution range | ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 60kDa band observed in Human Liver lysates (calculated MW of 58.9kDa according to NP_006648.1and NP_996848.1). Recommended concentration: 0.01-0.03 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti FTCD antibody, anti LCHC1 antibody, anti form Read more... |
| Note | For research use only |
| Entrez | 10841 |
0.75 µg/ml staining of Human (A) and Mouse (B) and (0.5 ug/ml) of Rat Liver lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
6 µg/ml staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
