GLUT2 Rabbit Polyclonal Antibody

• Catalog Number: orb183810
• Category: Antibodies
• Description: GLUT2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Equine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GLUT2 (1-100/524aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: SLC2A2
• UniProt ID: P11168
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GTR2_HUMAN; Solute carrier family 2, facilitated g
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4°C). Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (orb183810), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-GLUT2 (orb183810) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.

Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Kidney (Mouse) Lysate at 40 ug, Lane 3: Pancreas (Mouse) Lysate at 40 ug, Primary: Anti-GLUT2 (orb183810) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57/45 kD, Observed band size: 47 kD.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-GLUT2 (orb183810) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUT2 Polyclonal Antibody, Unconjugated (orb183810) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.