GFP Mouse Monoclonal Antibody

• Catalog Number: orb499984
• Category: Antibodies
• Description: GFP Mouse Monoclonal Antibody
• Target: GFP
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: All
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant Green Fluorescence Protein
• Tested applications: WB
• Dilution range: WB=1:50000-500000
• Antibody Type: Primary Antibody
• Clone Number: 4G6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Green fluorescence protein;
• Note: For research use only

Sample: GFP-Tagged Fusion Protein Overexpression E.coli Lysate (orb1516874) at 2 ug, Primary: Anti-GST tag (orb499984) at 1/5000 ~ 1/10000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD.

Sample: GFP-tagged fusion protein Overexpression E.coli Lysate (orb1516874) at 4 ug, Primary: Anti-GFP-Tag (orb499984) at 1/1000000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 30 kD.

Transformed (GFP or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with orb499984 (Anti-GFP) at dilution of 1:1000000 incubated at 4°C overnight.