General Tetrahydrobiopterin (THB) ELISA Kit

• Catalog Number: orb1946757
• Category: Assays and Kits
• Description: General Tetrahydrobiopterin (THB) ELISA Kit
• Target: THB
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tetrahydrobiopterin(THB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tetrahydrobiopterin(THB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tetrahydrobiopterin(THB) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 100 ng/mL
• Range: 1.57-100ng/mL
• Sensitivity: 0.51 ng/mL
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: Tetrahydrobiopterin; BH4; Kuvan; Sapropterin
• Research Area: Disease Biomarkers
• Note: For research use only