General Substance P (SP) ELISA Kit

• Catalog Number: orb1806566
• Category: Assays and Kits
• Description: General Substance P (SP) ELISA Kit
• Target: SP
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Substance PKit(SP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Substance PKit(SP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Substance PKit(SP) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 5000pg/mL
• Range: 78.13-5000pg/mL
• Sensitivity: 46.88 pg/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: TAC1;TAC2;NKNA
• Research Area: Metabolism Research, Neuroscience
• Note: For research use only
• Expiration Date: Please enquire.