General Stercobilin (SB) ELISA Kit

• Catalog Number: orb782113
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Stercobilin(SB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Stercobilin(SB).
• Reactivity: All
• Concentration: 100 ng/mL
• Tested applications: ELISA
• Application notes: standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SB in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.58 ng/mL
• Sample Types: serum, plasma, stool and other biological fluids
• Alternative names: SB
• Research Area: Metabolism Research
• Note: For research use only
• Expiration Date: Please enquire.