General Sphingosine-1-Phosphate (S1P) ELISA Kit

• Catalog Number: orb782102
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sphingosine-1-Phosphate(S1P) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingosine-1-Phosphate(S1P).
• Reactivity: All
• Concentration: 1000 ng/mL
• Tested applications: ELISA
• Application notes: standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with S1P protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S1P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S1P in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 15.63-1000 ng/mL
• Sensitivity: 4.48 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: Sphingosine-1-Phosphate ELISA Kit
• Research Area: Disease Biomarkers
• Note: For research use only
• Expiration Date: Please enquire.