General Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit

• Catalog Number: orb781857
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Nicotinic Acid Adenine Dinucleotide Phosphate(NAADP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nicotinic Acid Adenine Dinucleotide Phosphate(NAADP).
• Reactivity: All
• Concentration: 10000 ng/mL
• Tested applications: ELISA
• Application notes: standard: 10000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NAADP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NAADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NAADP in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 156.25-10000 ng/mL
• Sensitivity: 51 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: NAADP
• Research Area: Cancer Biology, Metabolism Research, Protein Bioch
• Note: For research use only
• Expiration Date: Please enquire.