General Heparan Sulfate (HS) ELISA Kit

• Catalog Number: orb782193
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HS(Heparan Sulfate) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HS(Heparan Sulfate).
• Reactivity: All
• Concentration: 20 ng/mL
• Tested applications: ELISA
• Application notes: standard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HS in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Alternative names: HS ELISA Kit, Heparan Sulfate ELISA Kit
• Research Area: Cancer Biology, Infectious Disease & Virology, Met
• Note: For research use only