General Free Testosterone (F-Testo) ELISA Kit

• Catalog Number: orb1146748
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with FreeTestosterone(F-TESTO) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FreeTestosterone(F-TESTO). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FreeTestosterone(F-TESTO) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: All
• Concentration: 2000 pg/mL
• Application notes: standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F-TESTO protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F-TESTO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-TESTO in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 18.76 pg/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: F-TESTO
• Research Area: Metabolism Research, Stem Cell & Developmental Bio
• Note: For research use only
• Expiration Date: Please enquire.