General Estrone Sulfate (E1S) ELISA Kit

• Catalog Number: orb781765
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Estrone Sulfate(E1S) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Estrone Sulfate(E1S).
• Reactivity: All
• Concentration: 100 ng/mL
• Tested applications: ELISA
• Application notes: standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with E1S protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E1S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E1S in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.51 ng/mL
• Sample Types: Serum, plasma and other biological fluids.
• Alternative names: E1S
• Research Area: Stem Cell & Developmental Biology
• Note: For research use only
• Expiration Date: Please enquire.