General Cortisol (Cor) ELISA Kit

• Catalog Number: orb1806542
• Category: Assays and Kits
• Description: General Cortisol (Cor) ELISA Kit
• Target: Cor
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cortisol(Cor) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cortisol(Cor). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cortisol(Cor) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 20ng/mL
• Range: 0.31-20ng/mL
• Sensitivity: 0.19 ng/mL
• Sample Types: serum, plasma, urine and other biological fluids
• Alternative names: Hydrocortisone
• Research Area: Metabolism Research
• Note: For research use only
• Expiration Date: Please enquire.