General Citrulline (Cit) ELISA Kit

• Catalog Number: orb1088248
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Citrulline(Cit) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Citrulline(Cit). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Citrulline(Cit) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: All
• Concentration: 100 ng/mL
• Application notes: standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cit protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cit. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cit in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.49 ng/mL
• Sample Types: serum, plasma, urine and other biological fluids
• Alternative names: Citrulline
• Research Area: Metabolism Research
• Note: For research use only
• Expiration Date: Please enquire.