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Item 1 of 2
GAPDH Antibody, HRP Conjugate
Catalog Number: orb1926019
| Catalog Number | orb1926019 |
|---|---|
| Category | Antibodies |
| Description | Purified Mouse Monoclonal Antibody (Mab) |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 1653CT401.3.33 |
| Tested applications | WB |
| Reactivity | Human |
| Isotype | IgG1 |
| Dilution range | WB: 1:40000, WB: 1:1000 |
| Form/Appearance | Purified monoclonal antibody conjugated with HRP supplied in PBS with 0.05% (W/V) Proclin 300. This antibody is purified through a protein G column, followed by size-exclusion - Membrane filtration purification. |
| Conjugation | Unconjugated |
| MW | 36053 Da |
| Target | This GAPDH antibody is generated from a mouse immunized with a recombinant protein of human GAPDH. |
| UniProt ID | P04406 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | GAPD Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-GAPDH Antibody, HRP Conjugate at 1:40000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-GAPDH Antibody (conjugated HRP) at 1:1000 dilution. Lane 1: NIH/3T3 whole cell lysate. Lane 1: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 36kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
