GABA-B Receptor 2 Antibody: Biotin

• Catalog Number: orb182095
• Category: Antibodies
• Description: Mouse monoclonal antibody against GABA-B Receptor 2, conjugated to Biotin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N81/2 (Formerly sold as S81-2)
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Fusion protein amino acids 861-912 of rat GABA(B)R2
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: Biotin
• MW: 105kDa
• Target: GABA B Receptor 2
• Entrez: 83633
• UniProt ID: O88871
• NCBI: NP_113990.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: BcDNA:GH07312 antibody, CG6706 antibody, CT20836 a
• Note: For research use only
• Application notes: 1 µg/ml of SMC-402 was sufficient for detection of GABA(B)R2 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody, Clone N81/2. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 2 Antibody (D) Composite.

Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA B Receptor 2 protein using Mouse Anti-GABA B Receptor 2 Monoclonal Antibody, Clone N81/2. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 μg). Load: 10 μg. Block: 5% milk. Primary Antibody: Mouse Anti-GABA B Receptor 2 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.