FIS1 Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1152053
• Category: Antibodies
• Description: FIS1 Recombinant Mouse Monoclonal Antibody
• Target: FIS1
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: IgG1, Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: KLH conjugated synthetic peptide derived from human FIS1
• UniProt ID: Q9Y3D6
• MW: 17 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CGI 135; CGI135; FIS 1; FIS1; Fis1 homolog; FIS1,
• Research Area: Apoptosis, Cell Biology, Cell Cycle, Metabolism, M
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: room temperature 2h, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: MCF-7, 2: T47D, 3: MDA-MB-231, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 15 kDa, Observed MW: 15 kDa.

Cell line: HepG2, Fixative: 4% Paraformaldehydel, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152053.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (FIS1) monoclonal Antibody, Unconjugated (orb1152053) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.