F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin conjugated

• Catalog Number: orb348337
• Category: Antibodies
• Description: F(ab')2 Rat IgG (H&L) antibody (RPE)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG F(ab')2
• Conjugation: RPE
• Reactivity: Rat
• Form/Appearance: Lyophilized
• Concentration: 0.5 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Immunogen: Rat IgG whole molecule
• Tested applications: FC, IF, WB
• Dilution range: FC: 1:100 - 1:250, IF: 1:100 - 1:250
• Application notes: F(ab')2 Anti-Rat IgG Phycoerythrin Conjugated Antibody has been tested by western blot and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Alternative names: Goat F(ab')2 Anti-Rat IgG Phycoerythrin Conjugated
• Note: For research use only
• Expiration Date: 12 months from date of receipt.