F(ab')2 Rabbit IgG (H&L) Antibody Rhodamine Conjugated

• Catalog Number: orb348290
• Category: Antibodies
• Description: F(ab')2 Rabbit IgG (H&L) antibody (TRITC)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG F(ab')2
• Conjugation: TRITC
• Reactivity: Rabbit
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Immunogen: Rabbit IgG whole molecule
• Tested applications: DOT, IF
• Dilution range: IF: 1:500-1:2,500
• Application notes: F(ab')2 Anti-Rabbit IgG Rhodamine Conjugated Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Goat F(ab')2 Anti-Rabbit IgG Rhodamine Conjugated
• Note: For research use only

Dot Blot of F(ab')2 Goat anti-Rabbit IgG Antibody Rhodamine Conjugated. Antigen: Rabbit IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: F(ab')2 Goat anti-Rabbit IgG Antibody Rhodamine Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT.