F(ab')2 Rabbit IgG Fc Antibody Biotin Conjugated

• Catalog Number: orb348313
• Category: Antibodies
• Description: F(ab')2 Rabbit IgG F(c) antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG F(ab')2
• Conjugation: Biotin
• Reactivity: Rabbit
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: F(ab') fragment biotin conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Rabbit IgG F(ab).
• Immunogen: Rabbit IgG F(c) fragment
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: F(ab')2 Anti-Rabbit IgG F(c) Biotin Antibody has been tested by SDS-PAGE and western blot and is assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Goat F(ab')2 Anti-Rabbit IgG F(c) Biotin Conjugate
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot of Goat anti-F(ab')2 Rabbit IgG F(c) Antibody Biotin Conjugated Lane 1: Rabbit Fc Load: 100 ng per lane Primary antibody: F(ab')2 Rabbit IgG F(c) Antibody Biotin Conjugated at 1:1000 for overnight at 4°C Secondary antibody: HRP Streptavidin at 1:40000 for 30 min at RT Block: orb348637 for 30 min RT Predicted/Observed size: 28 kDa, 28 kDa