F(ab')2 Guinea Pig IgG (H&L) Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb348140
• Category: Antibodies
• Description: F(ab')2 Guinea Pig IgG (H&L) antibody (Alkaline Phosphatase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG F(ab')2
• Conjugation: AP
• Reactivity: Guinea pig
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1 mg/mL
• Buffer/Preservatives: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
• Immunogen: Guinea Pig IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500-1:1,000, WB: 1:1,000-1:5,000
• Application notes: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Guinea pig IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: Rabbit F(ab')2 Anti-Guinea Pig IgG Antibody Alkali
• Note: For research use only