F(ab')2 Golden Syrian Hamster IgG (H&L) Antibody Texas Red Conjugated

• Catalog Number: orb348151
• Category: Antibodies
• Description: F(ab')2 Golden Syrian Hamster IgG (H&L) antibody (Texas Red)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, FLISA, IF, WB
• Reactivity: Golden Hamster
• Isotype: IgG F(ab')2
• Immunogen: Golden Syrian Hamster IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:500-1:2,500
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). Limited reactivity will occur against Armenian Hamster IgG.
• Conjugation: Texas Red
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit F(ab')2 Anti-Golden Syrian & Armenian Hamst
• Note: For research use only
• Application notes: F(ab')2 Anti-Golden Syrian Hamster IgG Texas Red Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Western Blot of Texas Red™ conjugated Rabbit F(ab')2 Anti-Golden Syrian Hamster IgG secondary antibody. Lane 1: Golden Syrian Hamster IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red™ rabbit F(ab')2 secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min t RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Golden Syrian Hamster IgG. Other band(s): None.