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F(ab')2 Dog IgG (H&L) Antibody Texas Red Conjugated
Catalog Number: orb348105
| Catalog Number | orb348105 |
|---|---|
| Category | Antibodies |
| Description | F(ab')2 Dog IgG (H&L) antibody (Texas Red) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, FLISA, IF, WB |
| Reactivity | Canine |
| Isotype | IgG F(ab')2 |
| Immunogen | Dog IgG whole molecule |
| Antibody Type | Secondary Antibody |
| Concentration | 1.0 mg/mL |
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:500-1:2,500 |
| Form/Appearance | Lyophilized |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Dog IgG and Dog Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). |
| Conjugation | Texas Red |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | Rabbit F(ab')2 Anti-Dog IgG Antibody Texas Red Con Read more... |
| Note | For research use only |
| Application notes | F(ab')2 Anti-Dog IgG Texas Red Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit anti-Dog IgG Texas Red Conjugated Antibody. Lane 1: Dog IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red rabbit secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Dog IgG. Other band(s): None.
