ERG Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1499398
• Category: Antibodies
• Description: ERG Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 2F3
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ERG
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: ERG
• UniProt ID: P11308
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ERG_HUMAN; Transcriptional regulator ERG; ERG; Tra
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug total protein per lane of various lysates probed with ERG monoclonal antibody, unconjugated (orb1499398) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Cell line: THP-1, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1499398.

Cell line: THP-1, Fixation: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary Ab Dilution: 1:100, Secondary Ab: Goat Anti-Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1499398.

Tissue: Human kidney, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499398.

Tissue: Human prostatic cancer, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499398.

Tissue: Mouse cerebrum, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499398.

Tissue: Rat kidney, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499398.