EpCAM / CD326 (Epithelial Marker) Antibody

• Catalog Number: orb750871
• Category: Antibodies
• Description: Mouse monoclonal antibody against EpCAM conjugated to Biotin
• Target: EPCAM
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Biotin
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab conjugated to Biotin
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Purification: Protein G
• Immunogen: Small cell lung carcinoma cells
• UniProt ID: P16422
• MW: 40-43kDa
• Tested applications: FC, IF, IHC
• Dilution range: Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood); Immunofluorescence (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: HT29 cells or breast tumor
• Clone Number: VU-1D9
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-Adenocarcinoma-associated Antigen antibody, a
• Research Area: Stem Cell & Developmental Biology
• Note: For research use only
• Entrez: 4072
• Expiration Date: 12 months from date of receipt.

Flow Cytometry of HT29 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ep-CAM Monoclonal Antibody (SPM134).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Biotin-conjugated Ep-CAM Mouse Monoclonal Antibody (VU-1D9).