EGFR (phospho Tyr1197) rabbit pAb

• Catalog Number: orb764178
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EGFR (phospho-Tyr1197)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1197. AA range:1161-1210
• MW: 175
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-EGFR antibody, anti-ERBB antibody, anti-ERBB1
• Note: For research use only

Western Blot analysis of various cells using Phospho-EGFR (Y1197) Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of A431 cells using Phospho-EGFR (Y1197) Polyclonal Antibody diluted at 1:1000.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Tyr1197) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from A431 cells treated with EGF 40 muM 10', using EGFR (Phospho-Tyr1197) Antibody. The lane on the left is blocked with the phospho peptide.