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EGFR domain III Antibody [h-R3 (Nimotuzumab)], Rabbit IgG - Research Grade Biosimilar

Catalog Number: orb1671894

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$ 1,050.00
Catalog Numberorb1671894
CategoryAntibodies
DescriptionEGFR domain III Antibody [h-R3 (Nimotuzumab)], Rabbit IgG - Research Grade Biosimilar
ClonalityRecombinant
Clone Numberh-R3 (Nimotuzumab)
Tested applicationsBlocking, FC, IHC
ReactivityHuman
IsotypeIgG kappa
ImmunogenThis antibody we generated by humanisation of the murine antibody R3, raised against EGFR purified from placenta membranes and A431 tumors.
Antibody TypePrimary Antibody
Concentrationbatch dependent
ConjugationUnconjugated
TargetC3
UniProt IDP00533
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Alternative namesERBB-D3, ERBB1, HER1, PIG61, mENA, avian erythrobl
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NoteFor research use only
Expiration Date12 months from date of receipt.
EGFR domain III Antibody [h-R3 (Nimotuzumab)], Rabbit IgG - Research Grade Biosimilar

Immunofluorescence staining of fixed HeLa cells with anti-EGFR domain III antibody h-R3 (Nimotuzum723). Immunofluorescence analysis of unpermearaformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rsion of h-R3 (orb1671894) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671894- DAPI- merged channels and an isotype control. The isotype control was stained with unimmunised r followed by Alexa Fluor® 488 secondary antibody.