EGF Receptor / EGFR Antibody

• Catalog Number: orb2641194
• Category: Antibodies
• Description: This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GFR/2341
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 344-492 from the human protein was used as the immunogen for the EGFR antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This EGFR antibody is available for research use only.
• UniProt ID: P00533
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the EGFR antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

Western blot testing of A431 lysate using EGFR antibody (clone GFR/2341). Expected molecular weight: 134-180 kDa depending on glycosylation level.

IHC testing of FFPE human placenta with EGFR antibody (clone GFR/2341). Required HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE human lung squamous cell carcinoma with EGFR antibody (clone GFR/2341). Required HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

SDS-PAGE analysis of purified, BSA-free EGFR antibody (clone GFR/2341) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using EGFR antibody (clone GFR/2341). These results demonstrate the foremost specificity of the GFR/2341 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.