EEF1A1 Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1172823
• Category: Antibodies
• Description: EEF1A1 Recombinant Mouse Monoclonal Antibody
• Target: EEF1A1
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: IgG1, Kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: C2C12
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: DNA and RNA Research, Epigenetics and Nuclear Sign
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: MCF-7, Daudi, 4T1, BRL, Protein loading quantity: 20 µg, Exposure time: 1 s, Predicted MW: 50 kDa, Observed MW: 50 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: MCF-7, Daudi, 4T1, BRL, Protein loading quantity: 20 µg, Exposure time: 1 s, Predicted MW: 50 kDa, Observed MW: 50 kDa.

Cell line: MCF7, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172823.

Tissue: Human breast cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172823.

Tissue: Mouse brain, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172823.

Tissue: Rat liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172823.