dsDNA Antibody / Double Stranded DNA

• Catalog Number: orb750161
• Category: Antibodies
• Description: Deoxyribonucleic acid (DNA) is a nucleic acid that stores long-term information regarding the development and function of all known living organisms. DNA consists of two long nucleotide polymers, which are composed of four bases, namely adenine, thymine, guanine and cytosine, all of which are flanked by a phosphate-deoxyribose backbone. This mAb can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG3, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Cell nuclei were used as the immunogen for the dsDNA antibody.
• UniProt ID: Not Known
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
• Application notes: Titering of the dsDNA antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: DRNC1-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

IHC testing of FFPE human tonsil with dsDNA antibody (clone DRNC1-1). FFPE testing requires sections to be boiled in pH9 10 mM Tris with 1 mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

IHC testing of FFPE human colon carcinoma with dsDNA antibody (clone DRNC1-1). FFPE testing requires sections to be boiled in pH9 10 mM Tris with 1 mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.