Dicer Antibody: APC

• Catalog Number: orb150098
• Category: Antibodies
• Description: Mouse monoclonal to Dicer (APC). Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha, pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs. The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N167/7 (Formerly sold as S167-7)
• Tested applications: ICC, IF, IHC
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: APC
• MW: 215kDa
• Target: Dicer
• Entrez: 192119
• UniProt ID: Q8R418
• NCBI: NP_683750.2
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Alternative names: Dicer1 antibody, DCR antibody, DCR1 antibody, DCR-
• Note: For research use only
• Application notes: 1 µg/ml of SMC-416 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dicer Monoclonal Antibody, Clone N167/7. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Dicer Antibody (D) Composite.

Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone N167/7. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 μg. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.