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desmosome/hemidesmosome Antibody [F12], Rabbit IgG
Catalog Number: orb1671860
| Catalog Number | orb1671860 |
|---|---|
| Category | Antibodies |
| Description | desmosome/hemidesmosome Antibody [F12], Rabbit IgG |
| Clonality | Recombinant |
| Clone Number | F12 |
| Tested applications | IEM, IF |
| Reactivity | Human |
| Isotype | IgG kappa |
| Immunogen | F12 was established by fusing peripheral blood lymphocytes derived from a human vulgaris patient with a hetermyeloma cell line. |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Conjugation | Unconjugated |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
![desmosome/hemidesmosome Antibody [F12], Rabbit IgG](/images//pub/media/catalog/product/NewWebsite/15/orb1671860_1.jpg)
Flow-cytometry using the anti-desmosome/hemidesmosome antibody F12. A431 cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r-chimeric version of F12 (orb1671860 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
