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| Catalog Number | orb1172803 |
|---|---|
| Category | Antibodies |
| Description | DBR1 Recombinant Mouse Monoclonal Antibody |
| Target | DBR1 |
| Clonality | Recombinant |
| Species/Host | Mouse |
| Isotype | IgG2b/Kappa |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Purification | Affinity purified by Protein G |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Antibody Type | Primary Antibody |
| Clone Number | C11A2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Research Area | DNA and RNA Research, RNA Processing |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 4°C overnight, Lysate: 1: Rat heart, 2: Mouse heart, 3: HeLa, 4: A431, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 75 kDa, Observed MW: 75 kDa.

Tissue: Mouse brain, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1172803.

Tissue: Rat brain, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1172803.
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