Cytokeratin, Pan antibody (MSVA-000R)

• Catalog Number: orb2276600
• Category: Antibodies
• Description: Cytokeratin, Pan antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-000R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: Q01546, Q7Z794
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: K1B; KRT1B; Keratin, type II cytoskeletal 1b; K77;
• Note: For research use only
• Application notes: Positive Control: Liver: A strong staining of all bile duct epithelial cells and an at least moderate, predominantly membranous staining of most hepatocytes should be seen. Negative Control: Tonsil: All lymphocytes must not stain. However, interstitial reticulum cells with dendritic/reticular pattern show a weak to moderate fibrillar staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Invasive lobular breast cancer showing strong diffuse CKpan positivity in tumor cells.

Liver In the liver a strong cytoplasmic staining of all bile ductal epithelial cells and an at least moderate predominantly membranous immunostaining of hepatocytes is seen.

Tonsil, surface epithelium - Squamous epithelial cells are strongly CKpan positive while all lymphocytes are CKpan negative.