Cytokeratin 20 Antibody / CK20

• Catalog Number: orb640111
• Category: Antibodies
• Description: This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: KRT20/3129R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A recombinant human partial protein (amino acids 196-323) was used as the immunogen for this Cytokeratin 20 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant CK20 antibody is available for research use only.
• UniProt ID: P35900
• Storage: Store the recombinant CK20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon with recombinant CK20 antibody (clone KRT20/3129R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma with recombinant CK20 antibody (clone KRT20/3129R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HT29 cell lysate with recombinant CK20 antibody (clone KRT20/3129R). Predicted molecular weight ~46 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant CK20 antibody (clone KRT20/3129R) as confirmation of integrity and purity.