Cytokeratin 18 Antibody

• Catalog Number: orb534403
• Category: Antibodies
• Description: Keratin 18 (KRT18) is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. [Wiki]
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Human full length recombinant protein was used as the immunogen for the recombinant Keratin 18 antibody.
• UniProt ID: P05783
• Tested applications: FACS, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Application notes: The stated application concentrations are suggested starting points. Titration of the recombinant Keratin 18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: CTKN18-2R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only

Western blot testing of human HCT116 antibody with recombinant Keratin 18 antibody (clone CTKN18-2R). Predicted molecular weight ~48 kDa.

IHC staining of FFPE human colon carinoma with recombinant Keratin 18 antibody (clone CTKN18-2R). Required HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 10-20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Keratin 18 antibody (clone CTKN18-2R); Red = isotype control, Blue = recombinant Keratin 18 antibody.